DNA Duplex Formation with a Coarse-Grained Model

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• 作者：Maciej Maciejczyk ; Aleksandar Spasic ; Adam Liwo ; Harold A. Scheraga
• 刊名：Journal of Chemical Theory and Computation
• 出版年：2014
• 出版时间：November 11, 2014
• 年：2014
• 卷：10
• 期：11
• 页码：5020-5035
• 全文大小：689K
• ISSN：1549-9626

文摘

A middle-resolution coarse-grained model of DNA is proposed. The DNA chain is built of spherical and planar rigid bodies connected by elastic virtual bonds. The bonded part of the potential energy function is fit to potentials of mean force of model systems. The rigid bodies are sets of neutral, charged, and dipolar beads. Electrostatic and van der Waals interactions are parametrized by our recently developed procedure [Maciejczyk, M.; Spasic, A.; Liwo, A.; Scheraga, H.A. J. Comp. Chem. 2010, 31, 1644]. Interactions with the solvent and an ionic cloud are approximated by a multipole鈥搈ultipole Debye鈥揌眉ckel model. A very efficient R-RATTLE algorithm, for integrating the movement of rigid bodies, is implemented. It is the first coarse-grained model, in which both bonded and nonbonded interactions were parametrized ab initio and which folds stable double helices from separated complementary strands, with the final conformation close to the geometry of experimentally determined structures.