Mass Spectrometry-Assisted Protease Substrate Screening

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• 作者：Hartmut Schl&uuml ; ter ; Jana Rykl ; Joachim Thiemann ; Sandra Kurzawski ; Johan Gobom ; Martin Tepel ; Walter Zidek ; Michael Linscheid
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：February 1, 2007
• 年：2007
• 卷：79
• 期：3
• 页码：1251 - 1255
• 全文大小：212K
• 年卷期：v.79,no.3(February 1, 2007)
• ISSN：1520-6882

文摘

Since sequencing of the human genome was completed,more than 500 genes have been annotated as proteases.Exploring the physiological role of each protease requiresthe identification of their natural substrates. However, theendogenous substrates of many of the human proteasesare as yet unknown. Here we describe a new assay thataddresses this problem. The assay, which easily can beautomated, is based on the incubation of immobilizedprotein fractions, which may contain the natural substrate,with a defined protease. After concentrating the proteolytically released peptides by reversed-phase chromatographythey are analyzed by tandem mass spectrometry and thesubstrates identified by database searching. The proof ofprinciple in this study is demonstrated by incubatingimmobilized human plasma proteins with thrombin andby identifying by tandem mass spectrometry the fibrinopeptides, released by the action of thrombin from theirnatural substrate fibrinogen, in the reaction mixture.