Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein鈥揚rotein Binding Affinities
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- 作者：Rub茅n Ahijado-Guzm谩n ; Janak Prasad ; Christina Rosman ; Andreas Henkel ; Lydia Tome ; Dirk Schneider ; Germ谩n Rivas ; Carsten S枚nnichsen
- 刊名：Nano Letters
- 出版年：2014
- 出版时间：October 8, 2014
- 年：2014
- 卷：14
- 期：10
- 页码：5528-5532
- 全文大小：316K
- ISSN：1530-6992
文摘
Most of current techniques used for the quantification of protein鈥損rotein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.