文摘
Most of current techniques used for the quantification of protein鈥損rotein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
Keywords:
NanoSPR; plasmonic nanosensors; multiple protein鈭抪rotein interactions; optical dark-field spectroscopy; FtsZ