Spectroscopy of Non-Heme Iron Thiolate Complexes: Insight into the Electronic Structure of the Low-Spin Active Site of Nitrile Hydratase
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- 作者：Pierre Kennepohl ; Frank Neese ; Dirk Schweitzer ; Henry L. Jackson ; Julie A. Kovacs ; and Edward I. Solomon
- 刊名：Inorganic Chemistry
- 出版年：2005
- 出版时间：March 21, 2005
- 年：2005
- 卷：44
- 期：6
- 页码：1826 - 1836
- 全文大小：448K
- 年卷期：v.44,no.6(March 21, 2005)
- ISSN：1520-510X
文摘
Detailed spectroscopic and computational studies of the low-spin iron complexes [FeIII(S2Me2N3(Pr,Pr))(N3)] (1) and[FeIII(S2Me2N3(Pr,Pr))]1+ (2) were performed to investigate the unique electronic features of these species and theirrelation to the low-spin ferric active sites of nitrile hydratases. Low-temperature UV/vis/NIR and MCD spectra of 1and 2 reflect electronic structures that are dominated by antibonding interactions of the Fe 3d manifold and theequatorial thiolate S 3p orbitals. The six-coordinate complex 1 exhibits a low-energy S
Fe 3dxy (~13000 cm-1)charge-transfer transition that results predominantly from the low energy of the singly occupied Fe 3dxy orbital, dueto pure interactions between this acceptor orbital and both thiolate donor ligands in the equatorial plane. The3d 3d
ligand-field transitions in this species occur at higher energies (>15000 cm-1), reflecting its near-octahedral symmetry. The Fe 3dxz,yz Fe 3dxy (d d) transition occurs in the near-IR and probes the FeIII-S-donor bond; this transition reveals vibronic structure that reflects the strength of this bond (
e 
340 cm-1). Incontrast, the ligand-field transitions of the five-coordinate complex 2 are generally at low energy, and the S Fecharge-transfer transitions occur at much higher energies relative to those in 1. This reflects changes in thiolatebonding in the equatorial plane involving the Fe 3dxy and Fe 3dx2-y2 orbitals. The spectroscopic data lead to asimple bonding model that focuses on the and interactions between the ferric ion and the equatorial thiolateligands, which depend on the S-Fe-S bond angle in each of the complexes. These electronic descriptions provideinsight into the unusual S = 1/2 ground spin state of these complexes: the orientation of the thiolate ligands inthese complexes restricts their -donor interactions to the equatorial plane and enforces a low-spin state. Theseanisotropic orbital considerations provide some intriguing insights into the possible electronic interactions at theactive site of nitrile hydratases and form the foundation for further studies into these low-spin ferric enzymes.
Fe 3dxy (~13000 cm-1)charge-transfer transition that results predominantly from the low energy of the singly occupied Fe 3dxy orbital, dueto pure interactions between this acceptor orbital and both thiolate donor ligands in the equatorial plane. The3d 3d ligand-field transitions in this species occur at higher energies (>15000 cm-1), reflecting its near-octahedral symmetry. The Fe 3dxz,yz Fe 3dxy (d d) transition occurs in the near-IR and probes the FeIII-S-donor bond; this transition reveals vibronic structure that reflects the strength of this bond (e 340 cm-1). Incontrast, the ligand-field transitions of the five-coordinate complex 2 are generally at low energy, and the S Fecharge-transfer transitions occur at much higher energies relative to those in 1. This reflects changes in thiolatebonding in the equatorial plane involving the Fe 3dxy and Fe 3dx2-y2 orbitals. The spectroscopic data lead to asimple bonding model that focuses on the and interactions between the ferric ion and the equatorial thiolateligands, which depend on the S-Fe-S bond angle in each of the complexes. These electronic descriptions provideinsight into the unusual S = 1/2 ground spin state of these complexes: the orientation of the thiolate ligands inthese complexes restricts their -donor interactions to the equatorial plane and enforces a low-spin state. Theseanisotropic orbital considerations provide some intriguing insights into the possible electronic interactions at theactive site of nitrile hydratases and form the foundation for further studies into these low-spin ferric enzymes.