Oxygen Sensing Difluoroboron β-Diketonate Polylactide Materials with Tunable Dynamic Ranges for Wound Imaging

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• 作者：Christopher A. DeRosa ; Scott A. Seaman ; Alexander S. Mathew ; Catherine M. Gorick ; Ziyi Fan ; James N. Demas ; Shayn M. Peirce ; Cassandra L. Fraser
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：November 23, 2016
• 年：2016
• 卷：1
• 期：11
• 页码：1366-1373
• 全文大小：587K
• ISSN：2379-3694

文摘

Difluoroboron β-diketonate poly(lactic acid) materials exhibit both fluorescence (F) and oxygen sensitive room-temperature phosphorescence (RTP). Introduction of halide heavy atoms (Br and I) is an effective strategy to control the oxygen sensitivity in these materials. A series of naphthyl-phenyl (nbm) dye derivatives with hydrogen, bromide, and iodide substituents were prepared for comparison. As nanoparticles, the hydrogen derivative was hypersensitive to oxygen (0–0.3%), while the bromide analogue was suited for hypoxia detection (0–3% O₂). The iodo derivative, BF₂nbm(I)PLA, showed excellent F to RTP peak separation and a 0–100% oxygen sensitivity range, unprecedented for metal-free RTP emitting materials. Due to the dual emission and exceptionally long RTP lifetimes of these O₂ sensing materials, a portable, cost-effective camera was used to quantify oxygen levels via lifetime and red/green/blue (RGB) ratiometry. The hypersensitive H dye was well matched to lifetime detection; simultaneous lifetime and ratiometric imaging was possible for the bromide analogue, whereas the iodide material, with intense RTP emission and a shorter lifetime, was suited for RGB ratiometry. To demonstrate the prospects of this camera/material design combination for bioimaging, iodide boron dye-PLA nanoparticles were applied to a murine wound model to detect oxygen levels. Surprisingly, wound oxygen imaging was achieved without covering (i.e., without isolating from ambient conditions, air). Additionally, wound healing was monitored via wound size reduction and associated oxygen recovery, from hypoxic to normoxic. These single-component materials provide a simple tunable platform for biological oxygen sensing that can be deployed to spatially resolve oxygen in a variety of environments.