Structure and Phase Behavior of Archaeal Lipid Monolayers
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- 作者：Christoph Jeworrek ; Florian Evers ; Mirko Erlkamp ; Sebastian Grobelny ; Metin Tolan ; Parkson Lee-Gau Chong ; Roland Winter
- 刊名：Langmuir
- 出版年：2011
- 出版时间：November 1, 2011
- 年：2011
- 卷：27
- 期：21
- 页码：13113-13121
- 全文大小：955K
- 年卷期：v.27,no.21(November 1, 2011)
- ISSN：1520-5827
文摘
We report X-ray reflectivity (XRR) and grazing incidence X-ray diffraction (GIXD) measurements of archaeal bipolar tetraether lipid monolayers at the air鈥搘ater interface. Specifically, Langmuir films made of the polar lipid fraction E (PLFE) isolated from the thermoacidophilic archaeon Sulfolobus acidocaldarius grown at three different temperatures, i.e., 68, 76, and 81 掳C, were examined. The dependence of the structure and packing properties of PLFE monolayers on surface pressure were analyzed in a temperature range between 10 and 50 掳C at different pH values. Additionally, the interaction of PLFE monolayers (using lipids derived from cells grown at 76 掳C) with the ion channel peptide gramicidin was investigated as a function of surface pressure. A total monolayer thickness of approximately 30 脜 was found for all monolayers, hinting at a U-shaped conformation of the molecules with both head groups in contact with the interface. The monolayer thickness increased with rising film pressure and decreased with increasing temperature. At 10 and 20 掳C, large, highly crystalline domains were observed by GIXD, whereas at higher temperatures no distinct crystallinity could be observed. For lipids derived from cells grown at higher temperatures, a slightly more rigid structure in the lipid dibiphytanyl chains was observed. A change in the pH of the subphase had an influence only on the structure of the lipid head groups. The addition of gramicidin to an PLFE monolayer led to a more disordered state as observed by XRR. In GIXD measurements, no major changes in lateral organization could be observed, except for a decrease of the size of crystalline domains, indicating that gramicidin resides mainly in the disordered areas of the monolayer and causes local membrane perturbation, only.