Stimulus-Responsive Hydrogels Made From Biosynthetic Fibrinogen Conjugates for Tissue Engineering: Structural Characterization
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- 作者：Ilya Frisman ; Yonatan Shachaf ; Dror Seliktar ; Havazelet Bianco-Peled
- 刊名：Langmuir
- 出版年：2011
- 出版时间：June 7, 2011
- 年：2011
- 卷：27
- 期：11
- 页码：6977-6986
- 全文大小：1194K
- 年卷期：v.27,no.11(June 7, 2011)
- ISSN：1520-5827
文摘
Nanostructured hydrogels based on 鈥渟mart鈥?polymer conjugates of poloxamers and protein molecules were developed in order to form stimulus-responsive materials with bioactive properties for 3-D cell culture. Functionalized Pluronic F127 was covalently attached to a fibrinopeptide backbone and cross-linked into a structurally versatile and mechanically stable polymer network endowed with bioactivity and temperature-responsive structural features. Small angle X-ray scattering and transmission electron microscopy combined with rheology were used to characterize the structural and mechanical features of this biosynthetic conjugate, both in solution and in hydrogel form. The temperature at which the chemical cross-linking of F127-fibrinopeptide conjugates was initiated had a profound influence on the mechanical properties of the thermo-responsive hydrogel. The analysis of the scattering data revealed modification in the structure of the protein backbone resulting from increases in ambient temperature, whereas the structure of the polymer was not affected by ambient temperature. The hydrogel cross-linking temperature also had a major influence on the modulus of the hydrogel, which was rationally correlated to the molecular structure of the polymer network. The hydrogel structure exhibited a small mesh size when cross-linked at low temperatures and a larger mesh size when cross-linked at higher temperatures. The mesh size was nicely correlated to the mechanical properties of the hydrogels at the respective cross-linking temperatures. The schematic charts that model this material鈥檚 behavior help to illustrate the relationship that exists between the molecular structure, the cross-linking temperature, and the temperature-responsive features for this class of protein鈥損olymer conjugates. The precise control over structural and mechanical properties that can be achieved with this bioactive hydrogel material is essential in designing a tissue-engineering scaffold for clinical applications.