Lipidomic 鈥淒eep Profiling鈥? An Enhanced Workflow to Reveal New Molecular Species of Signaling Lipids
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- 作者：Pradeep Narayanaswamy ; Sudhirkumar Shinde ; Robert Sulc ; Rachel Kraut ; Gregory Staples ; Chung Hwee Thiam ; Rudolf Grimm ; B枚rje Sellergren ; Federico Torta ; Markus R. Wenk
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：March 18, 2014
- 年：2014
- 卷：86
- 期：6
- 页码：3043-3047
- 全文大小：367K
- 年卷期：v.86,no.6(March 18, 2014)
- ISSN：1520-6882
文摘
Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.