Lab-on-a-Disc for Fully Integrated Multiplex Immunoassays

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• 作者：Jiwoon Park ; Vijaya Sunkara ; Tae-Hyeong Kim ; Hyundoo Hwang ; Yoon-Kyoung Cho
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：March 6, 2012
• 年：2012
• 卷：84
• 期：5
• 页码：2133-2140
• 全文大小：446K
• 年卷期：v.84,no.5(March 6, 2012)
• ISSN：1520-6882

文摘

This paper presents a cost-effective, rapid, and fully automated lab-on-a-disc for simultaneous detection of multiple protein biomarkers in raw samples such as whole blood or whole saliva. For the diagnosis of cardiovascular disease, here, a novel centrifugal microfluidic layout was designed to conduct the simultaneous detection of high sensitivity C-reactive protein, cardiac troponin I, and N-terminal pro-B type natriuretic peptide based on a bead-based sandwich type enzyme-linked immunosorbent assay (ELISA). Three reaction chambers are initially interconnected for the common processes such as sample injection, incubation, and washing and then isolated on-demand for the independent processes such as substrate incubation and final detection. The assay performances such as the limit of detection and the dynamic range were comparable with those of the conventional ELISA despite the significant reduction of the minimum sample volume (200 渭L), the amount of washing buffer (700 渭L), and the total process time (20 min).