Direct-Write Patterning of Bacterial Cells by Dip-Pen Nanolithography
详细信息 查看全文
- 作者：Jieun Kim ; Young-Hun Shin ; Seong-Hun Yun ; Dong-Sik Choi ; Ji-Hye Nam ; Sung Ryong Kim ; Sung-Kwon Moon ; Bong Hyun Chung ; Jae-Hyuck Lee ; Jae-Ho Kim ; Ki-Young Kim ; Kyung-Min Kim ; Jung-Hyurk Lim
- 刊名：The Journal of the American Chemical Society
- 出版年：2012
- 出版时间：October 10, 2012
- 年：2012
- 卷：134
- 期：40
- 页码：16500-16503
- 全文大小：218K
- 年卷期：v.134,no.40(October 10, 2012)
- ISSN：1520-5126
文摘
The ability of dip-pen nanolithography (DPN) to generate nano- or microarrays of soft or hard materials (e.g., small molecules, DNA, proteins, nanoparticles, sols, and polymers) in a direct-write manner has been widely demonstrated. The transporting of large-sized ink materials such as bacteria, however, remains a significant challenge with this technique. The size limitation of the water meniscus formed between the DPN tip and the solid surface becomes a bottleneck in such diffusion-based molecular transport experiments. Herein, we report a straightforward 鈥渟tamp-on鈥?DPN method that uses a nanostructured poly(2-methyl-2-oxazoline) hydrogel-coated tip and carrier agents to generate patterns of micrometer-sized Escherichia coli JM 109 bacterial cells. We demonstrate that this approach enables the deposition of a single bacterial cell array on a solid surface or arrays of layers of multiple cells by modulating the viscosity of the 鈥渋nk鈥?solution. Fluorescence microscopy images indicated that the deposited bacterial cells were kept alive on Luria鈥揃ertani-agar layered solid surfaces after DPN patterning.