Characterization and Distribution of Trace Organic Contaminants in Sediment from Masan Bay, Korea. 2. In Vitro Gene Expression Assays

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• 作者：Jong Seong Khim ; Daniel L. Villeneuve ; Kurunthachalam Kannan ; Chul Hwan Koh ; and John P. Giesy
• 刊名：Environmental Science & Technology
• 出版年：1999
• 出版时间：December 1, 1999
• 年：1999
• 卷：33
• 期：23
• 页码：4206 - 4211
• 全文大小：85K
• 年卷期：v.33,no.23(December 1, 1999)
• ISSN：1520-5851

文摘

Extracts of sediment collected from Masan Bay, Koreawere fractionated using Florisil columns. Fractions werescreened for their ability to induce estrogen- and dioxinlikegene expression in vitro using MVLN and H4IIE-luc cells,respectively, both before and after acid treatment. Florisilfraction 1 (F1), which was shown to contain polychlorinatedbiphenyls, induced very little response in either assay.The midpolarity Florisil fraction (F2) was the most activefraction. Twenty-seven of 28 F2 samples induced significantestrogenic activity, and all 28 samples induced significantdioxinlike activity. Twelve of the F2 samples producedmagnitudes of response in the dioxin-responsive H4IIE-luccells similar to those induced by a 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) standard. Acid treatment did not markedlydiminish the activity of F2 samples. These results suggestedthe presence of unidentified, acid stable, aryl hydrocarbonreceptor (AhR) and estrogen receptor (ER) agonists in F2.Twenty-three of the 28 most polar florisil fractions (F3)were either cytotoxic or caused morphological changesin estrogen-responsive MVLN cells, while over half of theF3 samples caused similar effects in H4IIE-luc cells.Empirical evidence suggested that acid labile compoundscontributed to both the estrogenic and cytotoxic responsesof the MVLN cells. Mass balance suggested that knownconcentrations of alkylphenols and bisphenol A mayaccount for a portion of the estrogenic response but werenot great enough to account for the toxicity. Acid labilecompounds also contributed substantially to the dioxinlikeactivity of F3 samples. This adds to a growing body ofevidence which suggests the presence of unidentified,relatively polar, aryl hydrocarbon receptor agonists in sedimentfrom some areas.