Response to Cardiac Markers in Human Serum Analyzed by Guided-Mode Resonance Biosensor

详细信息    查看全文

• 作者：Wan-Joong Kim ; Bong Kyu Kim ; Ansoon Kim ; Chul Huh ; Chil Seong Ah ; Kyung-Hyun Kim ; Jongcheol Hong ; Seon Hee Park ; Sanghoon Song ; Junghan Song ; Gun Yong Sung
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：December 1, 2010
• 年：2010
• 卷：82
• 期：23
• 页码：9686-9693
• 全文大小：457K
• 年卷期：v.82,no.23(December 1, 2010)
• ISSN：1520-6882

文摘

Cardiac markers in human serum with concentrations less than 0.1 ng/mL were analyzed by use of a guided-mode resonance (GMR) biosensor. Cardiac troponin I (cTnI), creatine kinase MB (CK-MB), and myoglobin (MYO) were monitored in the serum of both patients and healthy controls. Dose−response curves ranging from 0.05 to 10 ng/mL for cTnI, from 0.1 to 10 ng/mL for CK-MB, and from 0.03 to 1.7 μg/mL for MYO were obtained. The limits of detection (LOD) for cTnI, CK-MB, and MYO were less than 0.05, 0.1, and 35 ng/mL, respectively. Analysis time was 30 min, which is short enough to meet clinical requirements. Antibody immobilization and the hydrophilic properties of the guided-mode resonance filter (GMRF) surface were investigated by X-ray photoelectron spectroscopy (XPS) and by monitoring the peak wavelength shift and water contact angle (CA). Both assays used to evaluate the surface density of the immobilized antibodies, a sandwich enzyme-linked immunosorbent assay (ELISA) and a sandwich immunogold assay, showed that the antibodies were successfully immobilized and sufficiently aligned to detect the low concentration of biomarkers. Our results show that the GMR biosensor will be very useful in developing low-cost portable biosensors that can screen for cardiac diseases.