Dynamic Copper(I) Imaging in Mammalian Cells with a Genetically Encoded Fluorescent Copper(I) Sensor

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• 作者：Seraphine V. Wegner ; Hasan Arslan ; Murat Sunbul ; Jun Yin ; Chuan He
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：March 3, 2010
• 年：2010
• 卷：132
• 期：8
• 页码：2567-2569
• 全文大小：172K
• 年卷期：v.132,no.8(March 3, 2010)
• ISSN：1520-5126

文摘

Copper, a key cofactor for many life processes, is toxic at elevated levels, and its availability is strictly controlled inside cells. Therefore, it is a challenge to visualize copper availability in the tight copper-binding environment of the cell. We report a genetically encoded fluorescent copper(I) sensor based on the copper(I)-binding-induced conformational change of a copper-responsive transcriptional regulator, Amt1. The resulting reporter, Amt1-FRET, is ratiometric, highly sensitive (K_d = 2.5 × 10⁻¹⁸ M), and selective toward copper(I). Its measured high affinity to copper(I) confirms the extremely low copper availability in yeast since Amt1 senses the upper limit of cellular copper levels in yeast and activates copper detoxification genes. Amt1-FRET operates in the dynamic range of the cellular copper buffer in mammalian cells and can report dynamic fluctuations of the cellular copper availability within minutes of perturbation. Thus, Amt1-FRET visualizes the tightly controlled copper availability in mammalian cells.