Isolation of Bioactive Compounds from Sunflower Leaves (Helianthus annuus L.) Extracted with Supercritical Carbon Dioxide

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• 作者：Zouhir El Marsni ; Ascension Torres ; Rosa M. Varela ; Jos茅 M. G. Molinillo ; Lourdes Casas ; Casimiro Mantell ; Enrique J. Martinez de la Ossa ; Francisco A. Macias
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 22, 2015
• 年：2015
• 卷：63
• 期：28
• 页码：6410-6421
• 全文大小：587K
• ISSN：1520-5118

文摘

The work described herein is a continuation of our initial studies on the supercritical fluid extraction (SFE) with CO₂ of bioactive substances from Helianthus annuus L. var. Arianna. The selected SFE extract showed high activity in the wheat coleoptile bioassay, in Petri dish phytotoxicity bioassays, and in the hydroponic culture of tomato seeds. Chromatographic fractionations of the extracts and a spectroscopic analysis of the isolated compounds showed 52 substances belonging to 10 different chemical classes, which were mainly sesquiterpene lactones, diterpenes, and flavonoids. Heliannuol M (31), helivypolides K and L (36, 37), and helieudesmanolide B (38) are described for the first time in the literature. Metabolites have been tested in the etiolated wheat coleoptile bioassay with good results in a noteworthy effect on germination. The most active compounds were also tested on tomato seeds, heliannuol A (30) and leptocarpin (45) being the most active, with values similar to those of the commercial herbicide.