Dipeptidyl Peptidase IV-Inhibitory Peptides Derived from Silver Carp (Hypophthalmichthys molitrix Val.) Proteins

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• 作者：Ying Zhang ; Ran Chen ; Xiling Chen ; Zhu Zeng ; Huiqin Ma ; Shangwu Chen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：February 3, 2016
• 年：2016
• 卷：64
• 期：4
• 页码：831-839
• 全文大小：485K
• ISSN：1520-5118

文摘

The dipeptidyl peptidase IV (DPP-IV)-inhibitory bioactivity of silver carp protein (SCP) hydrolysates were investigated, and their containing efficacious DPP-IV-inhibitory peptides were explored by in silico hydrolysis analysis, peptide separation combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS) identification, and chemical synthesis. SCP hydrolysates generated by six proteases all showed efficient DPP-IV-inhibitory activities, and Neutrase-generated hydrolysates had the greatest DPP-IV inhibition (IC₅₀ of 1.12 mg/mL). In silico Neutrase hydrolysis revealed hundreds of fragments released from myosin, actin, and collagen of SCPs, which include different Pro-motif peptides but only three reported peptidic DPP-IV inhibitors with moderate or weak bioactivity. In addition, three new DPP-IV-inhibitory peptides were identified using LC–MS/MS; in particular, LPIIDI and APGPAGP showed high DPP-IV-inhibitory activity with IC₅₀ of 105.44 and 229.14 μM, respectively, and behaved in competitive/non-competitive mixed-type DPP-IV inhibition mode. The results indicate that the SCP-derived DPP-IV-inhibitory peptides could be potential functional ingredients in the diabetic diet.