Rapid and Noninvasive Metabonomic Characterization of Inflammatory Bowel Disease
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- 作者：Julian R. Marchesi ; Elaine Holmes ; Fatima Khan ; Sunil Kochhar ; Pauline Scanlan ; Fergus Shanahan ; Ian D. Wilson ; Yulan Wang
- 刊名：Journal of Proteome Research
- 出版年：2007
- 出版时间：February 2007
- 年：2007
- 卷：6
- 期：2
- 页码：546 - 551
- 全文大小：192K
- 年卷期：v.6,no.2(February 2007)
- ISSN：1535-3907
文摘
Inflammatory bowel diseases (IBD) including Crohn's disease (CD) and ulcerative colitis (UC) have amajor impact on the health of individuals and populations. Accurate diagnosis of inflammatory boweldisease (IBD) at an early stage, and correct differentiation between Crohn's disease (CD) and ulcerativecolitis (UC), is important for optimum treatment and prognosis. We present here the first characterizationof fecal extracts obtained from patients with CD and UC by employing a noninvasive metabonomicsapproach, which combines high resolution 1H NMR spectroscopy and multivariate pattern recognitiontechniques. The fecal extracts of both CD and UC patients were characterized by reduced levels ofbutyrate, acetate, methylamine, and trimethylamine in comparison with a control population, suggestingchanges in the gut microbial community. Also, elevated quantities of amino acids were present in thefeces from both disease groups, implying malabsorption caused by the inflammatory disease or anelement of protein losing enteropathy. Metabolic differences in fecal profiles were more marked in theCD group in comparison with the control group, indicating that the inflammation caused by CD is moreextensive in comparison with UC and involves the whole intestine. Furthermore, glycerol resonanceswere a dominant feature of fecal spectra from patients with CD but were present in much lower intensityin the control and UC groups. This work illustrates the potential of metabonomics to generate novelnoninvasive diagnostics for gastrointestinal diseases and may further our understanding of diseasemechanisms.