Ordered Mesoporous Polymer−Silica Hybrid Nanoparticles as Vehicles for the Intracellular Controlled Release of Macromolecules

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• 作者：Tae-Wan Kim ; Igor I. Slowing ; Po-Wen Chung ; Victor Shang-Yi Lin
• 刊名：ACS Nano
• 出版年：2011
• 出版时间：January 25, 2011
• 年：2011
• 卷：5
• 期：1
• 页码：360-366
• 全文大小：378K
• 年卷期：0
• ISSN：1936-086X

文摘

A two-dimensional hexagonal ordered mesoporous polymer−silica hybrid nanoparticle (PSN) material was synthesized by polymerization of acrylate monomers on the surface of SBA-15 mesoporous silica nanoparticles. The structure of the PSN material was analyzed using a series of different techniques, including transmission electron microscopy, powder X-ray diffraction, and Nub>2ub> sorption analysis. These structurally ordered mesoporous polymer−silica hybrid nanoparticles were used for the controlled release of membrane-impermeable macromolecules inside eukaryotic cells. The cellular uptake efficiency and biocompatibility of PSN with human cervical cancer cells (HeLa) were investigated. Our results show that the inhibitory concentration (ICub>50ub>) of PSN is very high (>100 μg/mL per million cells), while the median effective concentration for the uptake (ECub>50ub>) of PSN is low (ECub>50ub> = 4.4 μg/mL), indicating that PSNs are fairly biocompatible and easily up-taken in vitro. A membrane-impermeable macromolecule, 40 kDa FITC-Dextran, was loaded into the mesopores of PSNs at low pH. We demonstrated that the PSN material could indeed serve as a transmembrane carrier for the controlled release of FITC-Dextran at the pH level inside live HeLa cells. We believe that further developments of this PSN material will lead to a new generation of nanodevices for intracellular controlled delivery applications.