Creating Aligned Arrays of Bacillus Megaterium in Sol-Gel Matrixes

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• 作者：Shantang Liu ; Lynn F. Wood ; Dennis E. Ohman ; Maryanne M. Collinson
• 刊名：Chemistry of Materials
• 出版年：2007
• 出版时间：May 29, 2007
• 年：2007
• 卷：19
• 期：11
• 页码：2752 - 2756
• 全文大小：438K
• 年卷期：v.19,no.11(May 29, 2007)
• ISSN：1520-5002

文摘

This paper describes a simple and convenient method for constructing an array of aligned bacteria andits immobilization in a solid framework. Paramagnetic particles were attached to Bacillus megateriumvia the carbodiimide coupling between the amine functional groups on the particle with carboxylic acidgroups on the bacteria. It was found that the amount of particles on the surface of the bacteria wasdependent on the experimental conditions such as reaction time. After the surface of the bacteria wasfully coated with the magnetic particles, the bacteria responded to an external magnetic force. By takingadvantage of this property, two-dimensional arrays of bacteria were easily formed on a glass substrateover a large area both in aqueous solution and in a silica-based sol. The aligned wire-like structures werefrozen in place via gelation of the sol. These structures are highly stable and reproducible and can beused to create nanostructured arrays on surfaces for the fabrication of novel chemical sensors or catalyticsupports.