Design of Cell-Permeable Stapled Peptides as HIV-1 Integrase Inhibitors

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• 作者：Ya-Qiu Long ; Shao-Xu Huang ; Zahrah Zawahir ; Zhong-Liang Xu ; Huiyuan Li ; Tino W. Sanchez ; Ying Zhi ; Stephanie De Houwer ; Frauke Christ ; Zeger Debyser ; Nouri Neamati
• 刊名：Journal of Medicinal Chemistry
• 出版年：2013
• 出版时间：July 11, 2013
• 年：2013
• 卷：56
• 期：13
• 页码：5601-5612
• 全文大小：542K
• 年卷期：v.56,no.13(July 11, 2013)
• ISSN：1520-4804

文摘

HIV-1 integrase (IN) catalyzes the integration of viral DNA into the host genome, involving several interactions with the viral and cellular proteins. We have previously identified peptide IN inhibitors derived from the 伪-helical regions along the dimeric interface of HIV-1 IN. Herein, we show that appropriate hydrocarbon stapling of these peptides to stabilize their helical structure remarkably improves the cell permeability, thus allowing inhibition of the HIV-1 replication in cell culture. Furthermore, the stabilized peptides inhibit the interaction of IN with the cellular cofactor LEDGF/p75. Cellular uptake of the stapled peptide was confirmed in four different cell lines using a fluorescein-labeled analogue. Given their enhanced potency and cell permeability, these stapled peptides can serve as not only lead IN inhibitors but also prototypical biochemical probes or 鈥渘anoneedles鈥?for the elucidation of HIV-1 IN dimerization and host cofactor interactions within their native cellular environment.