Conjugated-Polymer-Based Red-Emitting Nanoparticles for Two-Photon Excitation Cell Imaging with High Contrast

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• 作者：Shuang Li ; Xiaoqin Shen ; Lin Li ; Peiyan Yuan ; Zhenping Guan ; Shao Q. Yao ; Qing-Hua Xu
• 刊名：Langmuir
• 出版年：2014
• 出版时间：July 8, 2014
• 年：2014
• 卷：30
• 期：26
• 页码：7623-7627
• 全文大小：307K
• ISSN：1520-5827

文摘

Two-photon fluorescence microscopy is a widely used noninvasive bioimaging technique because of unique advantages such as a large penetration depth and 3D mapping capability. Ideal two-photon fluorophores require large two-photon absorption cross sections and red emission with high quantum yields. Here we report red-emitting-dye-doped conjugated polymer nanoparticles that display high two-photon excitation brightness. In these nanoparticles, conjugated polymer (PFV) was chosen as a two-photon light-harvesting material, and red-emitting dyes (MgPc and Nile red) were chosen as the energy acceptors and red-emitting materials. Two-photon excitation fluorescence of MgPc and Nile red was enhanced by up to 53 and 240 times, respectively. We have successfully demonstrated the application of these conjugated polymer-based nanoparticles in two-photon excitation cancer cell imaging with an excellent contrast ratio. This concept could become a general approach to the preparation of two-photon excitation red-emitting materials for deep-tissue live-cell imaging with high contrast.