Graphene Enhanced Electron Transfer at Aptamer Modified Electrode and Its Application in Biosensing

详细信息    查看全文

• 作者：Li Wang ; Miao Xu ; Lei Han ; Ming Zhou ; Chengzhou Zhu ; Shaojun Dong
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：September 4, 2012
• 年：2012
• 卷：84
• 期：17
• 页码：7301-7307
• 全文大小：376K
• 年卷期：v.84,no.17(September 4, 2012)
• ISSN：1520-6882

文摘

Graphene (GN), a two-dimensional and one-atom thick carbon sheet, is showing exciting applications because of its unique morphology and properties. In this work, a new electrochemical biosensing platform by taking advantage of the ultrahigh electron transfer ability of GN and its unique GN/ssDNA interaction was reported. Adenosine triphosphate binding aptamer (ABA) immobilized on Au electrode could strongly adsorb GN due to the strong 蟺鈥撓€ interaction and resulted in a large decrease of the charge transfer resistance (R_ct) of the electrode. However, the binding reaction between ABA and its target adenosine triphosphate (ATP) inhibited the adsorption of GN, and R_ct could not be decreased. On the basis of this, we developed a new GN-based biosensing platform for the detection of small molecule ATP. The experimental results confirmed that the electrochemical aptasensor we developed possessed a good sensitivity and high selectivity for ATP. The detection range for ATP was from 15 脳 10^鈥? to 4 脳 10^鈥? M. The method here was label-free and sensitive and did not require sophisticated fabrication. Furthermore, we can generalize this strategy to detect Hg²⁺ using a thymine (T)-rich, mercury-specific oligonucleotide. Therefore, we expected that this method may offer a promising approach for designing high-performance electrochemical aptasensors for the sensitive and selective detection of a spectrum of targets.