Hg2+ Removal by Genetically Engineered Escherichia coli in a Hollow Fiber Bioreactor
详细信息 查看全文
- 作者：ShaoLin Chen ; EunKi Kim ; Michael L. Shuler ; and David B. Wilson
- 刊名：Biotechnology Progress
- 出版年：1998
- 出版时间：October 1998
- 年：1998
- 卷：14
- 期：5
- 页码：667 - 671
- 全文大小：64K
- 年卷期：v.14,no.5(October 1998)
- ISSN：1520-6033
文摘
Escherichia coli cells engineered to express an Hg2+ transport system and metallothionein accumulated Hg2+ effectively over a concentration range of 0.2-4 mg/L inbatch systems. Bioaccumulation was selective against other metal ions and resistantto changes in ambient conditions such as pH, ionic strength, and the presence ofcommon metal chelators or complexing agents (Chen, S.-L.; Wilson, D. B. Appl.Environ. Microbiol. 1997, 63, 2442-2445; Biodegradation 1997, 8, 97-103). Herewe report the characterization of the bioaccumulation system based on its kineticsand an isotherm. Bioaccumulation was rapid and followed Michaelis-Menten kinetics.A hollow fiber bioreactor was constructed to retain the genetically engineered cells.The bioreactor was capable of removing and recovering Hg2+ effectively at lowconcentrations, reducing a 2 mg/L solution to about 5 
g/L. A mathematical equationthat quantitatively described Hg2+ removal by the bioreactor provides a basis for theoptimization and extrapolation of the bioreactor. The genetically engineered E. colicells and the bioreactor system have excellent properties for bioremediation of Hg2+-contaminated environments.
g/L. A mathematical equationthat quantitatively described Hg2+ removal by the bioreactor provides a basis for theoptimization and extrapolation of the bioreactor. The genetically engineered E. colicells and the bioreactor system have excellent properties for bioremediation of Hg2+-contaminated environments.