High-Salt-Tolerance Matrix for Facile Detection of Glucose in Rat Brain Microdialysates by MALDI Mass Spectrometry

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• 作者：Rui Chen ; Wenjun Xu ; Caiqiao Xiong ; Xiaoyu Zhou ; Shaoxiang Xiong ; Zongxiu Nie ; Lanqun Mao ; Yi Chen ; Huan-Cheng Chang
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：January 3, 2012
• 年：2012
• 卷：84
• 期：1
• 页码：465-469
• 全文大小：788K
• 年卷期：v.84,no.1(January 3, 2012)
• ISSN：1520-6882

文摘

Due to its strong ultraviolet absorption, high salt tolerance, and little interference in the low molecular weight region, N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) has been applied as a matrix to measure the level of glucose in rat brain microdialysates by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in combination with in vivo microdialysis. By monitoring the ion signals of (glucose + Cl)^{鈭?/sup> in the mass spectra, we achieved a low detection limit of 10 渭M for glucose in 126 mM NaCl, which is a typical component in artificial cerebrospinal fluid, without prior sample purification. It is concluded that NEDC-assisted laser desorption/ionization (LDI) MS is a fast and general method for sensitive detection of small molecules (such as glucose and amino acids) in high ionic strength solutions.}