Surface-Chemistry Effect on Cellular Response of Luminescent Plasmonic Silver Nanoparticles

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• 作者：Shasha Sun ; Chen Zhou ; Sishan Chen ; Jinbin Liu ; Jing Yu ; Jennifer Chilek ; Liang Zhao ; Mengxiao Yu ; Rodrigo Vinluan ; Bo Huang ; Jie Zheng
• 刊名：Bioconjugate Chemistry
• 出版年：2014
• 出版时间：March 19, 2014
• 年：2014
• 卷：25
• 期：3
• 页码：453-459
• 全文大小：426K
• 年卷期：v.25,no.3(March 19, 2014)
• ISSN：1520-4812

文摘

Cellular response of inorganic nanoparticles (NPs) is strongly dependent on their surface chemistry. By taking advantage of robust single-particle fluorescence and giant Raman enhancements of unique polycrystalline silver NPs (AgNPs), we quantitatively investigated effects of two well-known surface chemistries, passive PEGylation and active c-RGD peptide conjugation, on in vitro behaviors of AgNPs at high temporal and spatial resolution as well as chemical level using fluorescence and Raman microscopy. The results show that specific c-RGD peptide鈭捨?sub>v尾₃ integrin interactions not only induced endosome formation more rapidly, enhanced constrained diffusion, but also minimized nonspecific chemical interactions between the NPs and intracellular biomolecules than passive PEGylation chemistry; as a result, surface enhanced Raman scattering (SERS) signals of c-RGD peptides were well resolved inside endosomes in the live cells, while Raman signals of PEGylated AgNPs remained unresolvable due to interference of surrounding biomolecules, opening up an opportunity to investigate specific ligand鈥搑eceptor interactions in real time at the chemical level.