Open-Source Automated Parahydrogen Hyperpolarizer for Molecular Imaging Using 13C Metabolic Contrast Agents

详细信息    查看全文

• 作者：Aaron M. Coffey ; Roman V. Shchepin ; Milton L. Truong ; Ken Wilkens ; Wellington Pham ; Eduard Y. Chekmenev
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：August 16, 2016
• 年：2016
• 卷：88
• 期：16
• 页码：8279-8288
• 全文大小：747K
• 年卷期：0
• ISSN：1520-6882

文摘

An open-source hyperpolarizer producing ¹³C hyperpolarized contrast agents using parahydrogen induced polarization (PHIP) for biomedical and other applications is presented. This PHIP hyperpolarizer utilizes an Arduino microcontroller in conjunction with a readily modified graphical user interface written in the open-source processing software environment to completely control the PHIP hyperpolarization process including remotely triggering an NMR spectrometer for efficient production of payloads of hyperpolarized contrast agent and in situ quality assurance of the produced hyperpolarization. Key advantages of this hyperpolarizer include: (i) use of open-source software and hardware seamlessly allowing for replication and further improvement as well as readily customizable integration with other NMR spectrometers or MRI scanners (i.e., this is a multiplatform design), (ii) relatively low cost and robustness, and (iii) in situ detection capability and complete automation. The device performance is demonstrated by production of a dose (∼2–3 mL) of hyperpolarized ¹³C-succinate with %P_13C ∼ 28% and 30 mM concentration and ¹³C-phospholactate at %P_13C ∼ 15% and 25 mM concentration in aqueous medium. These contrast agents are used for ultrafast molecular imaging and spectroscopy at 4.7 and 0.0475 T. In particular, the conversion of hyperpolarized ¹³C-phospholactate to ¹³C-lactate in vivo is used here to demonstrate the feasibility of ultrafast multislice ¹³C MRI after tail vein injection of hyperpolarized ¹³C-phospholactate in mice.