Biomimetic Production of Silk-Like Recombinant Squid Sucker Ring Teeth Proteins

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• 作者：Dawei Ding ; Paul A. Guerette ; Shawn Hoon ; Kiat Whye Kong ; Tobias Cornvik ; Martina Nilsson ; Akshita Kumar ; Julien Lescar ; Ali Miserez
• 刊名：Biomacromolecules
• 出版年：2014
• 出版时间：September 8, 2014
• 年：2014
• 卷：15
• 期：9
• 页码：3278-3289
• 全文大小：679K
• ISSN：1526-4602

文摘

The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called 鈥渟uckerins鈥? which assemble into nanoconfined 尾-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to 尾-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in 尾-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications.