Characterization of the lnmKLM Genes Unveiling Key Intermediates for 尾-Alkylation in Leinamycin Biosynthesis

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• 作者：Yong Huang ; Sheng-Xiong Huang ; Jianhua Ju ; Gongli Tang ; Tao Liu ; Ben Shen
• 刊名：Organic Letters
• 出版年：2011
• 出版时间：February 4, 2011
• 年：2011
• 卷：13
• 期：3
• 页码：498-501
• 全文大小：209K
• 年卷期：v.13,no.3(February 4, 2011)
• ISSN：1523-7052

文摘

Leinamycin (LNM, 1) biosynthesis is proposed to involve 尾-alkylation of the polyketide intermediate, catalyzed by LnmKLM. Inactivation of lnmK, lnmL, or lnmM afforded mutant strains that accumulated LNM K-1 (2), K-2 (3), K-3 (4), and isomers LNM K-1鈥?(5), K-2鈥?(6), and K-3鈥?(7) whose polyketide origin was established by feeding experiments with sodium [1-¹³C]acetate. These findings confirm the indispensability of LnmKLM in 1 biosynthesis and suggest that 尾-alkylation proceeds on the growing polyketide intermediate while bound to the LNM polyketide synthase.