Mycoflora and Fumonisin Mycotoxins Associated with Cowpea [Vigna unguiculata (L.) Walp] Seeds
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- 作者：Quenton Kritzinger ; Theresa A. S. Aveling ; Walter F. O. Marasas ; John P. Rheeder ; Liana Van Der Westhuizen ; and Gordon S. Shephard
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2003
- 出版时间：April 9, 2003
- 年：2003
- 卷：51
- 期：8
- 页码：2188 - 2192
- 全文大小：59K
- 年卷期：v.51,no.8(April 9, 2003)
- ISSN：1520-5118
文摘
Cowpea seed samples from South Africa and Benin were analyzed for seed mycoflora. Fusariumspecies detected were F. equiseti, F. chlamydosporum, F. graminearum, F. proliferatum, F.sambucinum, F. semitectum, and F. subglutinans. Cowpea seed from South Africa and Benin and F.proliferatum isolates from Benin, inoculated onto maize patty medium, were analyzed for fumonisinproduction. Samples were extracted with methanol/water and cleaned up on strong anion exchangesolid phase extraction cartridges. HPLC with precolumn derivatization using o-phthaldialdehyde wasused for the detection and quantification of fumonisins. Cowpea cultivars from South Africa showedthe presence of fumonisin B1 at concentrations ranging between 0.12 and 0.61 
g/g, whereas thosefrom Benin showed no fumonisins. This is believed to be the first report of the natural occurrence ofFB1 on cowpea seed. Fumonisin B1, B2, and B3 were produced by all F. proliferatum isolates. Totalfumonisin concentrations were between 0.8 and 25.30 g/g, and the highest level of FB1 detectedwas 16.86 g/g.Keywords: Cowpea; fumonisins; FB1; Fusarium proliferatum; mycoflora; Vigna unguiculata
g/g, whereas thosefrom Benin showed no fumonisins. This is believed to be the first report of the natural occurrence ofFB1 on cowpea seed. Fumonisin B1, B2, and B3 were produced by all F. proliferatum isolates. Totalfumonisin concentrations were between 0.8 and 25.30 g/g, and the highest level of FB1 detectedwas 16.86 g/g.Keywords: Cowpea; fumonisins; FB1; Fusarium proliferatum; mycoflora; Vigna unguiculata