Production of Fumonisin B and C Analogues by Several Fusarium Species

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• 作者：Vikash Sewram ; Ndumiso Mshicileli ; Gordon S. Shephard ; Hester F. Vismer ; John P. Rheeder ; Yin-Won Lee ; John F. Leslie ; and Walter F. O. Marasas
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：June 15, 2005
• 年：2005
• 卷：53
• 期：12
• 页码：4861 - 4866
• 全文大小：69K
• 年卷期：v.53,no.12(June 15, 2005)
• ISSN：1520-5118

文摘

Six strains of Fusarium verticillioides, two of F. oxysporum, one strain of F. proliferatum, and a strainof an unidentified species were cultured on maize patties and rice and evaluated for their ability tosimultaneously produce fumonisin B (FB) and C (FC) series analogues. Fumonisins were quantifiedby LC-MS-MS using positive ion electrospray ionization. FC₁ provided characteristic fragment ionsat m/z 690, 672, 654, 532, 514, and 338 corresponding to sequential loss of H₂O and tricarboxylicacid moieties from the alkyl backbone, while FC₃ and FC₄ provided equivalent product ions 16 and32 amu lower than the corresponding FC₁ fragments, respectively. All isolates cultured on maizeproduced FC₄. All isolates except for that of F. proliferatum also produced FC₁, and three of the sixstrains of F. verticillioides produced FC₃. All isolates except those of F. oxysporum produced detectableamounts of FB₁, FB₂, and FB₃. Isolates that produced fumonisin B analogues produced at least 10fold more of the B series analogues than they did of the C series analogues. The results confirm thatat least some strains of F. oxysporum produce FC, but not FB, fumonisin analogues and also suggestthat the genetics and physiological regulation of fumonisin production may be more complicated thanpreviously envisaged since some strains of F. verticillioides and F. proliferatum as well as the strainof the unidentified species can simultaneously produce both FB and FC analogues.Keywords: Fusarium; fumonisins; tandem mass spectrometry; DNA; amplified fragment lengthpolymorphisms