Do Fumonisin Mycotoxins Occur in Wheat?
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- 作者：Gordon S. Shephard ; Liana van der Westhuizen ; Pumza M. Gatyeni ; David R. Katerere ; and Walter F. O. Marasas
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：November 16, 2005
- 年：2005
- 卷：53
- 期：23
- 页码：9293 - 9296
- 全文大小：45K
- 年卷期：v.53,no.23(November 16, 2005)
- ISSN：1520-5118
文摘
The fumonisin mycotoxins are mainly produced by the fungi Fusarium verticillioides and Fusariumproliferatum, which are both field pathogens of maize. The natural occurrence of fumonisins hasbeen verified in maize and a large range of maize-based products in many countries of the world.However, occasional reports have emerged of fumonisins being detected in wheat, despite the mainproducing fungi not being pathogens of this cereal. An investigation was conducted into a recentreport of the natural occurrence of fumonisins in the 2003/2004 South African wheat crop at levelsup to 1.7 mg/kg, as determined by immunoaffinity column cleanup and direct fluorometric measurement. An AOAC International high-performance liquid chromatographic (HPLC) method for thedetermination of fumonisins in maize was modified and validated for the determination of fumonisinsin spiked wheat samples. HPLC analysis of the wheat samples previously found to be positive forfumonisins revealed no detectable (<5 
g/kg) fumonisins in the 30 samples analyzed. These results,which lay doubt on previous reports of fumonisins in wheat, emphasize the fact that screening methods,especially if used outside their range or matrix of applicability, can produce false positive resultsdespite the use of immunoaffinity cleanup. Such results should be validated and confirmed with amore definitive technique.Keywords: Fumonisins; maize; wheat; Fusarium verticillioides; mycotoxins; HPLC
g/kg) fumonisins in the 30 samples analyzed. These results,which lay doubt on previous reports of fumonisins in wheat, emphasize the fact that screening methods,especially if used outside their range or matrix of applicability, can produce false positive resultsdespite the use of immunoaffinity cleanup. Such results should be validated and confirmed with amore definitive technique.Keywords: Fumonisins; maize; wheat; Fusarium verticillioides; mycotoxins; HPLC