Fast, Ratiometric FRET from Quantum Dot Conjugated Stabilized Single Chain Variable Fragments for Quantitative Botulinum Neurotoxin Sensing
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- 作者：Joonseok Lee ; Melissa B. Brennan ; Rosemarie Wilton ; Clare E. Rowland ; Elena A. Rozhkova ; Sara Forrester ; Daniel C. Hannah ; Julia Carlson ; Elena V. Shevchenko ; Daniel S. Schabacker ; Richard D. Schaller
- 刊名：Nano Letters
- 出版年：2015
- 出版时间：October 14, 2015
- 年：2015
- 卷：15
- 期：10
- 页码：7161-7167
- 全文大小：407K
- ISSN：1530-6992
文摘
Botulinum neurotoxin (BoNT) presents a significant hazard under numerous realistic scenarios. The standard detection scheme for this fast-acting toxin is a lab-based mouse lethality assay that is sensitive and specific, but slow (鈭? days) and requires expert administration. As such, numerous efforts have aimed to decrease analysis time and reduce complexity. Here, we describe a sensitive ratiometric fluorescence resonance energy transfer scheme that utilizes highly photostable semiconductor quantum dot (QD) energy donors and chromophore conjugation to compact, single chain variable antibody fragments (scFvs) to yield a fast, fieldable sensor for BoNT with a 20鈥?0 pM detection limit, toxin quantification, adjustable dynamic range, sensitivity in the presence of interferents, and sensing times as fast as 5 min. Through a combination of mutations, we achieve stabilized scFv denaturation temperatures of more than 60 掳C, which bolsters fieldability. We also describe adaptation of the assay into a microarray format that offers persistent monitoring, reuse, and multiplexing.