A Biological Strategy for Fabrication of Au/EGFP Nanoparticle Conjugates Retaining Bioactivity

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• 作者：Chiau-Yuang Tsai ; Ai-Li Shiau ; Pai-Chiao Cheng ; Dar-Bin Shieh ; Dong-Hwang Chen ; Chen-His Chou ; Chen-Sheng Yeh ; and Chao-Liang Wu
• 刊名：Nano Letters
• 出版年：2004
• 出版时间：July 2004
• 年：2004
• 卷：4
• 期：7
• 页码：1209 - 1212
• 全文大小：265K
• 年卷期：v.4,no.7(July 2004)
• ISSN：1530-6992

文摘

Recently, it has been found that thiolated oligonucleotides can be used to direct the assembly of gold colloids into ordered functionalnanostructures. However, approaches for fabricating Au/DNA conjugates by using a linear, functional, double-stranded (ds) DNA fragmentthat directly binds to a Au NP surface have not been reported. In this paper, we used a ligase-dependent strategy to synthesize a 1.7 kb,thiolated dsDNA fragment and generated novel nanocrystals by coupling it to 13 nm Au NPs. The conjugates contained Au/EGFP with EGFP/Au molar ratios of 1:1, 2:1, and 3:1, as determined by their electrophoretic mobility and AFM imaging. The Au/EGFP nanoconjugates could stillbe digested by restriction endonuclease (RE) and expressed as functional proteins in mammalian cells, indicating the biological activities ofDNA fragments were retained after conjugation with Au NPs. This biological strategy for fabrication of Au/DNA conjugates may be applied tomolecular imaging, nanomedicine, and nanobiosensor technology.