Survey of Differentially Expressed Proteins and Genes in Jasmonic Acid Treated Rice Seedling Shoot and Root at the Proteomics and Transcriptomics Levels

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• 作者：Kyoungwon Cho ; Ganesh Kumar Agrawal ; Junko Shibato ; Young-Ho Jung ; Yeon-Ki Kim ; Baek Hie Nahm ; Nam-Soo Jwa ; Shigeru Tamogami ; ^O ; Oksoo Han ; Kimiyoshi Kohda ; Hitoshi Iwahashi ; Randeep Rakwal
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：6
• 期：9
• 页码：3581 - 3603
• 全文大小：1028K
• 年卷期：v.6,no.9(September 2007)
• ISSN：1535-3907

文摘

Two global approaches were applied to develop an inventory of differentially expressed proteins andgenes in rice (cv. Nipponbare) seedling grown on Murashige and Skoog medium with and withoutjasmonic acid (JA). JA significantly reduced the growth of shoot, root, leaf, and leaf sheath dependingon JA concentration (1, 2, 5, 10, 25, and 50 M) as compared with control. Almost 50% growth inhibitionof seedling was observed with 5 M JA. Shoots and roots of seedlings grown on 5 M JA for 7 dayswere then used for proteomics and transcriptomics analyses. Two-dimensional gel electrophoresisrevealed 66 and 68 differentially expressed protein spots in shoot and root, respectively, compared totheir respective controls. Tandem mass spectrometry analysis of these proteins identified 52 (shoot)and 56 (root) nonredundant proteins, belonging to 10 functional categories. Proteins involved inphotosynthesis (44%), cellular respiratory (11%), and protein modification and chaperone (11%) werehighly represented in shoot, whereas antioxidant system (18%), cellular respiratory (17%), and defense-related proteins (15%) were highly represented in root. Transcriptomics analysis of shoot and rootidentified 107 and 325 induced genes and 34 and 213 suppressed genes in shoot and root, respectively.Except of unknown genes with over 57% of the total, most genes encode for proteins involved insecondary metabolism, energy production, protein modification and chaperone, transporters, andcytochrome P450. These identified proteins and genes have been discussed with respect to the JA-induced phenotype providing a new insight into the role of JA in rice seedling growth and development.