Gel-Based Proteomics of Unilateral Irradiated Striatum after Gamma Knife Surgery
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- 作者：Misato Hirano ; Randeep Rakwal ; Nobuo Kouyama ; Yoko Katayama ; Motohiro Hayashi ; Junko Shibato ; Yoko Ogawa ; Yasukazu Yoshida ; Hitoshi Iwahashi ; Yoshinori Masuo
- 刊名：Journal of Proteome Research
- 出版年：2007
- 出版时间：July 2007
- 年：2007
- 卷：6
- 期：7
- 页码：2656 - 2668
- 全文大小：768K
- 年卷期：v.6,no.7(July 2007)
- ISSN：1535-3907
文摘
Gamma knife surgery (GKS) is used for the treatment of various brain disorders. The biological effectsof focal gamma ray irradiation on targeted or surrounding areas in the brain are not well-known. Inthe present study, we evaluated protein expression changes in the unilateral irradiated (60 Gy) striatumin rat. Striata of irradiated and control brains were dissected 16 h post-irradiation for analysis by large-format two-dimensional gel electrophoresis (2-DGE). In parallel, we also examined the un-targetedcontralateral striatum over the control for potential changes in proteins patterns that may have occurreddue to the effects of irradiation to the unilateral striatum. A total of 17 reproducible and differentiallyexpressed silver nitrate-stained protein spots in the irradiated striatum was detected on 2-D gel. Theirsubsequent analysis by tandem mass spectrometry (nESI-LC-MS/MS) resulted in the identificationof 13 nonredundant proteins. Interestingly, out of these 13 changed proteins, 2 proteins were alsodetected in the contralateral striatum. Some of the significantly changed proteins identified were creatinekinase, protein disulfide isomerase A3 precursor (PDA3), and peroxiredoxin 2 (Prx2). Western analysiswith anti-PDA3 and anti-Prx2 antibodies revealed 4 and 2 cross-reacting protein spots on 2-D gel blots.Interestingly, after GKS, in the irradiated and un-irradiated striata, these spots showed a shift towardthe acidic side, suggesting post-translational modifications. Taken together, these results indicate thatunilateral irradiation during GKS triggers molecular changes in the bilateral striata.