Identification of Metabolites in Plasma and Urine of Uruguayan Propolis-Treated Rats
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- 作者：Shigenori Kumazawa ; Kayoko Shimoi ; Katsumi Hayashi ; Takeshi Ishii ; Tomoko Hamasaka ; and Tsutomu Nakayama
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：May 19, 2004
- 年：2004
- 卷：52
- 期：10
- 页码：3083 - 3088
- 全文大小：136K
- 年卷期：v.52,no.10(May 19, 2004)
- ISSN：1520-5118
文摘
Propolis is a resinous substance collected by honeybees from various plant sources. It is extensivelyused in food and beverages to improve health and prevent diseases such as heart disease, diabetes,and cancer. To investigate the absorption and metabolism of the components in propolis, in the presentstudy, we administered ethanol extracts of Uruguayan propolis (poplar type propolis) orally to ratsand analyzed their plasma and urine by high-performance liquid chromatography with photodiodearray and mass spectrometric detection. After deconjugation of the components by 
-glucuronidase/sulfatase treatment of the specimen, pinobanksin 5-methyl ether, pinobanksin, kaempferol, chrysin,pinocembrin, and galangin were detected in plasma of rats orally administered propolis. Thesecompounds were detected also in urine after -glucuronidase/sulfatase treatment. Furthermore,pinobanksin 5-methyl ether, pinobanksin, chrysin, pinocembrin, and galangin were present in theurine also in free form. These results suggest that flavonoids in propolis are metabolized and circulatein the body after oral administration of propolis.Keywords: Propolis; absorption; excretion; metabolites; flavonoid
-glucuronidase/sulfatase treatment of the specimen, pinobanksin 5-methyl ether, pinobanksin, kaempferol, chrysin,pinocembrin, and galangin were detected in plasma of rats orally administered propolis. Thesecompounds were detected also in urine after -glucuronidase/sulfatase treatment. Furthermore,pinobanksin 5-methyl ether, pinobanksin, chrysin, pinocembrin, and galangin were present in theurine also in free form. These results suggest that flavonoids in propolis are metabolized and circulatein the body after oral administration of propolis.Keywords: Propolis; absorption; excretion; metabolites; flavonoid