Integration of Multianalyte Sensing Functions on a Capillary-Assembled Microchip: Simultaneous Determination of Ion Concentrations and Enzymatic Activities by a "Drop-and-Sip" Technique

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• 作者：Terence G. Henares ; Masayuki Takaishi ; Naoya Yoshida ; Shigeru Terabe ; Fumio Mizutani ; Ryuichi Sekizawa ; Hideaki Hisamoto
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：February 1, 2007
• 年：2007
• 卷：79
• 期：3
• 页码：908 - 915
• 全文大小：340K
• 年卷期：v.79,no.3(February 1, 2007)
• ISSN：1520-6882

文摘

A general and simple implementation of simultaneousmultiparametric sensing in a single microchip is presented by using a capillary-assembled microchip (CAs-CHIP) integrated with the plural different reagent-releasecapillaries (RRCs), acting as various biochemical sensors.A novel "drop-and-sip" technique of fluid handling isperformed with a microliter droplet of a model samplesolution containing proteases (trypsin, chymotrypsin,thrombin, elastase) and divalent cations (Ca²⁺, Zn²⁺,Mg²⁺) that passes through the microchannel with the aidof a micropipette as a vacuum pump, concurrently fillingeach RRC via capillary force. To avert the evaporation ofthe nanoliter sample volume in each capillary, PDMS oilis dropped on the outlet hole of the CAs-CHIP exploitingthe capillary force that results in spontaneous sealing ofall the RRCs. In addition, this high-speed sample introduction alleviates the possibility of protein adsorption andcapillary cross-contamination, allowing a reliable andmultianalyte determination of a sample containing manydifferent proteases and divalent cations by using thefluorescence image analysis. Presented results suggestedthe possible application of this microchip in the field ofdrug discovery and systems biology.