Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)

详细信息    查看全文

• 作者：Bi-Feng Liu ; Motoaki Ozaki ; Yuichi Utsumi ; Tadashi Hattori ; and Shigeru Terabe
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：January 1, 2003
• 年：2003
• 卷：75
• 期：1
• 页码：36 - 41
• 全文大小：68K
• 年卷期：v.75,no.1(January 1, 2003)
• ISSN：1520-6882

文摘

Chemiluminescence (CL) detection integrated with amicrochip capillary electrophoresis (MCE) system thatwas fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two modelCL systems were involved here: metal ion-catalyzedluminol-peroxide reaction and dansyl species conjugatedperoxalate-peroxide reaction. Different strategies basedon three chip patterns (cross, cross combining with Y, andcross combining with V) to perform on-line CL detectionfor MCE were evaluated and compared in terms ofsensitivity, reproducibility, and peak symmetry. The chippattern of cross combining with Y proved to be promisingfor the luminol-peroxide CL system, while the chippattern of cross combining with V was preferred for theperoxalate-peroxide system where CL reagent could notbe effectively transported by electroosmotic flow. A detection limit down to submicromolar concentrations (midattomole) was achieved with good reproducibility andsymmetric peak shape. Successful separation of threemetal cations such as Cr(III), Co(II), and Cu(II) and chiralrecognition of dansyl phenylalanine enantiomers within1 min revealed distinct advantages of combining MCEwith CL detection for rapid and sensitive analyses.