Determination and Kinetics of Producing Glucosamine Using Fungi

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• 作者：Jui-Wei Hsieh ; Ho-Shing Wu ; Yu-Hong Wei ; Shaw S. Wang
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：23
• 期：5
• 页码：1009 - 1016
• 全文大小：793K
• 年卷期：v.23,no.5(October 2007)
• ISSN：1520-6033

文摘

This work used three fungi, Rhizopus oligosorus BCRC 31996, Monascus pilosus BCRC31527,and Aspergillus sp. BCRC31742, to produce glucosamine by using submerged fermentationand flask cultures. The reaction of glucosamine with 1-naphthyl isothiocyanate as derivatizingagent was carried out in pyridine at 50 C for 1 h. The derivative was accurately analyzed andquantified by using high performance liquid chromatography. The relative standard deviationof glucosamine determined between experimental and real values were less than 2%. The kineticand strategy of producing glucosamine in a flask culture was investigated to achieve an optimumyield of glucosamine under different conditions including three kinds of fungi, medium, and pHvalues. The descending ability of producing glucosamine for the three fungi was Aspergillus sp.BCRC31742 > Monascus pilosus BCRC31527 > Rhizopus oligosorus BCRC 31996 under theconditions studied. The experimental result shows that the glucosamine concentration had anoptimum value and was 3430 mg/L by using Aspergillus sp. BCRC31742 culture in glucoseand peptone (GP) medium, the yield of which was the best amount using wild-typemicroorganisms in the past. The generation culture of fungi and the pH control played importantroles in enhancing the yield of glucosamine. The specific growth rate of the microorganism andthe biomass, content, yield, and productivity of glucosamine were calculated as well.