文摘
Liquid chromatography coupled with electrospray mass spectrometry (LC-ESI/MS) with positive andnegative ion detection was used for the identification of flavonoids in Hakmeitau beans, a black seedcultivar of cowpea (Vigna sinensis). Gradient elution with water and acetonitrile, both containing 2%formic acid, was employed in chromatographic separation. The peaks were identified by comparisonof the retention times and the UV-vis spectroscopic and mass spectrometric data with authenticstandards and/or literature data. The identified flavonoids included six anthocyanins (cyanidin 3-O-galactoside, cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, malvidin 3-O-glucoside, peonidin 3-O-glucoside, and petunidin 3-O-glucoside) and four flavonol/flavonol glycosides (kaempferol 3-O-glucoside, quercetin, quercetin 3-O-glucoside, and quercetin 3-O-6' '-acetylglucoside). The tentativelyidentified flavonoids included two anthocyanins (malvidin 3-O-acetylglucoside and peonidin 3-O-malonylglucoside) and three flavonol glycosides (myricetin-3-O-glucoside, quercetin 7-O-glucoside,and quercetin-3-O-diglucoside). These flavonoids are present in seed coats, and the contents ofanthocyanins and flavonol glycosides were 20.7 and 2.0 mg/g, respectively.Keywords: Flavonoids; anthocyanins; flavonols; glycosides; Vigna sinensis; bean; LC-ESI/MS