Cell Surface-Anchored Fluorescent Aptamer Sensor Enables Imaging of Chemical Transmitter Dynamics
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- 作者：Takeshi Tokunaga ; Shigeyuki Namiki ; Katsuhiro Yamada ; Takahiro Imaishi ; Hiroshi Nonaka ; Kenzo Hirose ; Shinsuke Sando
- 刊名：The Journal of the American Chemical Society
- 出版年：2012
- 出版时间：June 13, 2012
- 年：2012
- 卷：134
- 期：23
- 页码：9561-9564
- 全文大小：298K
- 年卷期：v.134,no.23(June 13, 2012)
- ISSN：1520-5126
文摘
A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which allowed the direct anchoring of the fluorescent aptamer on the cell surface while retaining its performance as a fluorescent sensor. The fast-responsive fluorescent DNA aptamer sensor, which targets adenine compounds, was anchored on the surface of brain astrocytes. Fluorescence imaging of the aptamer-anchored astrocytes enabled the real-time monitoring of release of adenine compounds as a gliotransmitter, which was synchronized with calcium wave propagation in neighboring cells.