Nanogyroids Incorporating Multivalent Lipids: Enhanced Membrane Charge Density and Pore Forming Ability for Gene Silencing

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• 作者：Ceci虂lia Leal ; Kai K. Ewert ; Rahau S. Shirazi ; Nathan F. Bouxsein ; Cyrus R. Safinya
• 刊名：Langmuir
• 出版年：2011
• 出版时间：June 21, 2011
• 年：2011
• 卷：27
• 期：12
• 页码：7691-7697
• 全文大小：952K
• 年卷期：v.27,no.12(June 21, 2011)
• ISSN：1520-5827

文摘

The self-assembly of a custom-synthesized pentavalent cationic lipid (MVL5) and glycerol monooleate (GMO) with small interfering RNA (siRNA) results in the formation of a double-gyroid bicontinuous inverted cubic phase with colocalized lipid/siRNA domains as shown by synchrotron X-ray scattering and fluorescence microscopy. The high charge density (due to MVL5) and positive Gaussian modulus of the GMO-containing membranes confer optimal electrostatic and elastic properties for endosomal escape, enabling efficient siRNA delivery and effective, specific gene silencing.