Surface Enhanced Raman Scattering Detection of Cancer Biomarkers with Bifunctional Nanocomposite Probes
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- 作者：Jing Li ; Zakiya Skeete ; Shiyao Shan ; Shan Yan ; Katarzyna Kurzatkowska ; Wei Zhao ; Quang Minh Ngo ; Polina Holubovska ; Jin Luo ; Maria Hepel ; Chuan-Jian Zhong
- 刊名：Analytical Chemistry
- 出版年：2015
- 出版时间：November 3, 2015
- 年：2015
- 卷：87
- 期：21
- 页码：10698-10702
- 全文大小：348K
- ISSN：1520-6882
文摘
This report describes new findings of an investigation of a bifunctional nanocomposite probe for the detection of cancer biomarkers, demonstrating the viability of magnetic focusing and SERS detection in a microfluidic platform. The nanocomposite probe consists of a magnetic nickel鈥搃ron core and a gold shell. Upon bioconjugation, the nanoprobes are magnetically focused on a specific spot in a microfluidic channel, enabling an enrichment of 鈥渉ot spots鈥?for surface enhanced Raman scattering detection of the targeted carcinoembryonic antigen. The detection sensitivity, with a limit of detection of 鈭?.1 pM, is shown to scale with the magnetic focusing time and the nanoparticle size. The latter is also shown to exhibit an excellent agreement between the experimental data and the theoretical simulation. Implications of the findings to the development of rapid and sensitive microfluidic detection of cancer biomarkers are also discussed.