Wiring of Redox Enzymes on Three Dimensional Self-Assembled Molecular Scaffold

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• 作者：Marco Frasconi ; Arnon Heyman ; Izhar Medalsy ; Danny Porath ; Franco Mazzei ; Oded Shoseyov
• 刊名：Langmuir
• 出版年：2011
• 出版时间：October 18, 2011
• 年：2011
• 卷：27
• 期：20
• 页码：12606-12613
• 全文大小：940K
• 年卷期：v.27,no.20(October 18, 2011)
• ISSN：1520-5827

文摘

The integration of biological molecules and nanoscale components provides a fertile basis for the construction of hybrid materials of synergic properties and functions. Stable protein 1 (SP1), a highly stable ring shaped protein, was recently used to display different functional domains, to bind nanoparticles (NPs), and to spontaneously form two and three-dimensional structures. Here we show an approach to wire redox enzymes on this self-assembled protein鈥搉anoparticle hybrid. Those hybrids are genetically engineered SP1s, displaying glucose oxidase (GOx) enzymes tethered to the protein inner pore. Moreover, the Au-NP鈥損rotein hybrids self-assembled to multiple enzymatic layers on the surface. By wiring the redox enzymes to the electrode, we present an active structure for the bioelectrocatalytic oxidation of glucose. This system demonstrates for the first time a three-dimensional assembly of multiple catalytic modules on a protein scaffold with an efficient electrical wiring of the enzyme units on an electrode surface, thus implementing a hybrid electrically active unit for nanobioelectronic applications.