Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger
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- 作者：Emiel B. J. ten Buren ; Michiel A. P. Karrenbelt ; Marit Lingemann ; Shreyans Chordia ; Ying Deng ; JingJing Hu ; Johanna M. Verest ; Vincen Wu ; Teresita J. Bello Gonzalez ; Ruben G. A. van Heck ; Dorett I. Odoni ; Tom Schonewille ; Laura van der Straat ; Leo H. de Graaff ; Mark W. J. van Passel
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：December 19, 2014
- 年：2014
- 卷：3
- 期：12
- 页码：995-998
- 全文大小：198K
- ISSN：2161-5063
文摘
Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.