Structural Determination of Five Novel Tetrasaccharides Containing 3-O-Sulfated D-Glucuronic Acid and Two Rare Oligosaccharides Containing a 
详细信息 查看全文
- 作者：Akiko Kinoshita-Toyoda ; Shuhei Yamada ; Stuart M. Haslam ; Kay-Hooi Khoo ; Makiko Sugiura ; Howard R. Morris ; Anne Dell ; and Kazuyuki Sugahara
- 刊名：Biochemistry
- 出版年：2004
- 出版时间：August 31, 2004
- 年：2004
- 卷：43
- 期：34
- 页码：11063 - 11074
- 全文大小：352K
- 年卷期：v.43,no.34(August 31, 2004)
- ISSN：1520-4995
文摘
Oversulfated chondroitin sulfate E (CS-E) derived from squid cartilage exhibits intriguingbiological activities, which appear to reflect the biological activities of mammalian CS chains containingthe so-called E disaccharide unit [GlcA
1-3GalNAc(4,6-O-disulfate)]. Previously, we isolated novel tetra-and hexasaccharides containing a rare GlcA(3-O-sulfate) at the nonreducing end after digestion of squidcartilage CS-E with testicular hyaluronidase. In this study, squid cartilage CS-E was extensively digestedwith chondroitinase AC-II, which yielded five highly sulfated novel tetrasaccharides and two odd-numberedoligosaccharides (tri- and pentasaccharides) containing D-Glc. Their structures were determined by fastatom bombardment mass spectrometry and 1H NMR spectroscopy. The results revealed an internal GlcA(3-O-sulfate) residue for all the novel tetrasaccharide sequences, which rendered the oligosaccharidesresistant to the enzyme. The results suggest that GlcA(3-O-sulfate) units are not clustered but ratherinterspersed in the CS-E polysaccahride chains, being preferentially located in the highly sulfated sequences.The predominant structure on the nearest nonreducing side of a GlcA(3-O-sulfate) residue was GalNAc(4-O-sulfate) (80%), whereas that on the reducing side was GalNAc(4,6-O-disulfate) (59%). The structuralvariety in the vicinity of the GlcA(3-O-sulfate) residue might represent the substrate specificity of theunidentified chondroitin GlcA 3-O-sulfotransferase. The results also revealed a trisaccharide and apentasaccahride sequence, both of which contained a -D-Glc branch at the C6 position of the constituentGalNAc residue. Approximately 5 mol % of all disaccharide units were substituted by Glc in the CS-Epreparation used.
1-3GalNAc(4,6-O-disulfate)]. Previously, we isolated novel tetra-and hexasaccharides containing a rare GlcA(3-O-sulfate) at the nonreducing end after digestion of squidcartilage CS-E with testicular hyaluronidase. In this study, squid cartilage CS-E was extensively digestedwith chondroitinase AC-II, which yielded five highly sulfated novel tetrasaccharides and two odd-numberedoligosaccharides (tri- and pentasaccharides) containing D-Glc. Their structures were determined by fastatom bombardment mass spectrometry and 1H NMR spectroscopy. The results revealed an internal GlcA(3-O-sulfate) residue for all the novel tetrasaccharide sequences, which rendered the oligosaccharidesresistant to the enzyme. The results suggest that GlcA(3-O-sulfate) units are not clustered but ratherinterspersed in the CS-E polysaccahride chains, being preferentially located in the highly sulfated sequences.The predominant structure on the nearest nonreducing side of a GlcA(3-O-sulfate) residue was GalNAc(4-O-sulfate) (80%), whereas that on the reducing side was GalNAc(4,6-O-disulfate) (59%). The structuralvariety in the vicinity of the GlcA(3-O-sulfate) residue might represent the substrate specificity of theunidentified chondroitin GlcA 3-O-sulfotransferase. The results also revealed a trisaccharide and apentasaccahride sequence, both of which contained a -D-Glc branch at the C6 position of the constituentGalNAc residue. Approximately 5 mol % of all disaccharide units were substituted by Glc in the CS-Epreparation used.