Platforms Formed from a Three-Dimensional Cu-Based Zwitterionic Metal鈥揙rganic Framework and Probe ss-DNA: Selective Fluorescent Biosensors for Human Immunodeficiency Virus 1 ds-DNA and Sudan Virus RNA

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• 作者：Shui-Ping Yang ; Shao-Rui Chen ; Shu-Wen Liu ; Xiao-Yan Tang ; Liang Qin ; Gui-Hua Qiu ; Jin-Xiang Chen ; Wen-Hua Chen
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：December 15, 2015
• 年：2015
• 卷：87
• 期：24
• 页码：12206-12214
• 全文大小：649K
• ISSN：1520-6882

文摘

We herein report a water-stable three-dimensional Cu-based metal鈥搊rganic framework (MOF) 1 supported by a tritopic quaternized carboxylate and 4,4鈥?dipyridyl sulfide as an ancillary ligand. This MOF exhibits unique pore shapes with aromatic rings, positively charged pyridinium and unsaturated Cu(II) cation centers, free carboxylates, tessellating H₂O, and coordinating SO₄^{2鈥?/sup> on the pore surface. Compound 1 can interact with two carboxyfluorescein (FAM)-labeled single-stranded DNA sequences (probe ss-DNA, delineated as P-DNA) through electrostatic, 蟺-stacking, and/or hydrogen-bonding interactions to form two P-DNA@1 systems, and thus quench the fluorescence of FAM via a photoinduced electron-transfer process. These P-DNA@1 systems can be used as effective fluorescent sensors for human immunodeficiency virus 1 double-stranded DNA and Sudan virus RNA sequences, respectively, with detection limits of 196 and 73 pM, respectively.}