Preparation of Acetochlor Antibody and Its Application on Immunoaffinity Chromatography Cleanup for Residue Determination in Peanuts
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- 作者：Yiqiang Chen ; Xiong Wang ; Jianhua Wang ; Shusheng Tang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2009
- 出版时间：September 9, 2009
- 年：2009
- 卷：57
- 期：17
- 页码：7640-7643
- 全文大小：699K
- 年卷期：v.57,no.17(September 9, 2009)
- ISSN：1520-5118
文摘
The preparation of an antibody against acetochlor and its application on immunoaffinity chromatography (IAC) cleanup was investigated. The hapten was synthesized by reacting acetochlor with 3-mercaptopropionic acid and then linked with a carrier protein by the carbodiimide method. After immunizing a rabbit with hapten−bovine serum albumin (BSA) conjugate, the obtained antiserum showed an IC50 value of 0.2 ng/mL. By coupling the purified polyclonal antibody with CNBr-activated Sepharose 4B, an IAC column was prepared. The dynamic column capacity was 5560 ng/mL gel. The IAC was then used to clean up a peanut sample solution, followed by high-performance liquid chromatography (HPLC) separation and ultraviolet (UV) detection. The recoveries of acetochlor from spiked peanut samples at levels of 5−100 μg/kg ranged from 94.7 to 102.5%, with a variation coefficient of 2.6−8.0%, and the limits of detection and quantification were 1.5 and 5 μg/kg, respectively.