文摘
The preparation of an antibody against acetochlor and its application on immunoaffinity chromatography (IAC) cleanup was investigated. The hapten was synthesized by reacting acetochlor with 3-mercaptopropionic acid and then linked with a carrier protein by the carbodiimide method. After immunizing a rabbit with hapten−bovine serum albumin (BSA) conjugate, the obtained antiserum showed an IC50 value of 0.2 ng/mL. By coupling the purified polyclonal antibody with CNBr-activated Sepharose 4B, an IAC column was prepared. The dynamic column capacity was 5560 ng/mL gel. The IAC was then used to clean up a peanut sample solution, followed by high-performance liquid chromatography (HPLC) separation and ultraviolet (UV) detection. The recoveries of acetochlor from spiked peanut samples at levels of 5−100 μg/kg ranged from 94.7 to 102.5%, with a variation coefficient of 2.6−8.0%, and the limits of detection and quantification were 1.5 and 5 μg/kg, respectively.