Viscoelastic Properties and Dynamics of Porcine Gastric Mucin
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- 作者：Jonathan Celli ; Brian Gregor ; Bradley Turner ; Nezam H. Afdhal ; Rama Bansil ; and Shyamsunder Erramilli
- 刊名：Biomacromolecules
- 出版年：2005
- 出版时间：May 2005
- 年：2005
- 卷：6
- 期：3
- 页码：1329 - 1333
- 全文大小：98K
- 年卷期：v.6,no.3(May 2005)
- ISSN：1526-4602
文摘
Gastric mucin is a glycoprotein known to undergo a pH-dependent sol-gel transition that is crucial to theprotective function of the gastric mucus layer in mammalian stomachs. We present microscope-based dynamiclight scattering data on porcine gastric mucin at pH 6 (solution) and pH 2 (gel) with and without the presenceof tracer particles. The data provide a measurement of the microscale viscosity and the shear elastic modulusas well as an estimate of the mesh size of the gel formed at pH 2. We observe that the microscale viscosityin the gel is about 100-fold lower than its macroscopic viscosity, suggesting that large pores open up in thegel reducing frictional effects. The data presented here help to characterize physiologically relevant viscoelasticproperties of an important biological macromolecule and may also serve to shed light on diffusive motionof small particles in the complex heterogeneous environment of a polymer gel network.