Profiling and Characterization Antioxidant Activities in Anoectochilus formosanus Hayata

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• 作者：Sheng-Yang Wang ; Yueh-Hsiung Kuo ; Hsing-Ning Chang ; Pei-Ling Kang ; Hsin-Sheng Tsay ; Ku-Feng Lin ; Ning-Sun Yang ; and Lie-Fen Shyur
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：March 27, 2002
• 年：2002
• 卷：50
• 期：7
• 页码：1859 - 1865
• 全文大小：89K
• 年卷期：v.50,no.7(March 27, 2002)
• ISSN：1520-5118

文摘

Phytochemical characteristics and antioxidant activities of the crude and fractionated plant extractsof Anoectochilus formosanus were evaluated using five different assay systems. An acid-treatment(2 N HCl in 95% ethanol) was employed to treat a butanol fraction (BuOH), creating an acid-hydrolyzedBuOH fraction. The IC₅₀ values for DPPH radicals in the BuOH and acid-hydrolyzed BuOH fractionswere 0.521 and 0.021 mg/mL, respectively. The acid-hydrolyzed BuOH exhibited approximately 5-foldhigher activity in scavenging superoxide anion than catechin. The acid-hydrolyzed BuOH fractionalso effectively protected x174 supercoiled DNA against strand cleavage induced by H₂O₂ andreduced oxidative stress in HL-60 cells. Metabolite profiling showed that the aglycones of flavonoidglycosides in BuOH were produced after acid hydrolytic treatment, and this resulted in a significantincrease in antioxidant activities of acid-hydrolyzed BuOH. One new diarylpentanoid, kinsenone, andthree known flavonoid glycosides and their derivatives were identified for the first time from A.formosanus, with strong antioxidant properties.Keywords: Anoectochilus formosanus; antioxidant activity; acid treatment; flavonoid glycosides