TMIO-PyrImid Hybrids are Profluorescent, Site-Directed Spin Labels for Nucleic Acids

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• 作者：Benjamin A. Chalmers ; Subham Saha ; Tri Nguyen ; John McMurtrie ; Snorri Th. Sigurdsson ; Steven E. Bottle ; Kye-Simeon Masters
• 刊名：Organic Letters
• 出版年：2014
• 出版时间：November 7, 2014
• 年：2014
• 卷：16
• 期：21
• 页码：5528-5531
• 全文大小：328K
• ISSN：1523-7052

文摘

We report the synthesis of a new class of molecules which are hybrids of long-lived tetramethylisoindolinoxyl (TMIO) radicals and the pyrido[1,2-a]benzimidazole (PyrImid) scaffold. These compounds represent a new lead for noncovalently binding nucleic acid probes, as they interact with nucleic acids with previously unreported C (DNA) and C/U (RNA) complementarity, which can be detected by electron paramagnetic resonance (EPR) techniques. They also have promising properties for fluorimetric analysis, as their fluorescent spin-quenched derivatives exhibit a significant Stokes shift.